Generally, negative ions refer to ions in the air.
Historically, most research has been focused on air ions.
You cannot treat ions in water and those in the air in the same way.
Negative ions can disappear in the tubing connected to the pump before they reach the water.
Natural ores will not work.
Very few air ions can be created in water where there is little air.
Some ores can change the ratios of impurities or components contained in water as metal ions dissolve.
This may have some effects on fish but these effects are not caused by negative ions.